› 3ojo

X-ray diffraction
2.5Å resolution

Derivative structure of the UDP-N-acetyl-mannosamine dehydrogenase Cap5O from S. aureus

Released:
DOI: 10.2210/pdb3ojo/pdb
PDB entry 3ojo coloured by chain, front view.
PDB entry 3ojo coloured by chain, side view.
PDB entry 3ojo coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure analysis of the Staphylococcus aureus UDP-N-acetyl-mannosamine dehydrogenase Cap5O involved in capsular polysaccharide biosynthesis.
Gruszczyk J, Fleurie A, Olivares-Illana V, Béchet E, Zanella-Cleon I, Moréra S, Meyer P, Pompidor G, Kahn R, Grangeasse C, Nessler S
J Biol Chem 286 17112-21 (2011)
PMID: 21454499

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161175 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose/GDP-mannose dehydrogenase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 48.17 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P95708 (Residues: 2-420; Coverage: 100%)
Gene name: cap5O
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand NAD 2 x NAD
Ligand PDC 6 x PDC
Ligand EU 2 x EU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 40.22Å b: 131.497Å c: 158.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Bacterial tyrosine kinases: evolution, biological function and structural insights.
Grangeasse et al. (2012)
3 more

1 mention without citation

Structure analysis of the Staphylococcus aureus UDP-N-acetyl-mannosamine dehydrogenase Cap5O involved in capsular polysaccharide biosynthesis.
Gruszczyk et al. (2011)