3oeo

X-ray diffraction
2.7Å resolution

The crystal structure E. coli Spy

Released:
Source organism: Escherichia coli
Primary publication:
The crystal structure Escherichia coli Spy.
Protein Sci. 19 2252-9 (2010)
PMID: 20799348

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic chaperone Spy Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 138 amino acids
Theoretical weight: 15.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6ECL5 (Residues: 24-161; Coverage: 100%)
Gene names: ECBD_1902, spy
Sequence domains: LTXXQ motif family protein
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P32
Unit cell:
a: 69.195Å b: 69.195Å c: 126.347Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.252 0.3
Expression system: Escherichia coli BL21(DE3)