3oee

X-ray diffraction
2.74Å resolution

Structure of four mutant forms of yeast F1 ATPase: alpha-F405S

Released:
Source organism: Saccharomyces cerevisiae
Entry authors: Arsenieva D, Symersky J, Wang Y, Pagadala V, Mueller DM

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C, J, K, L, S, T, U
Molecule details ›
Chains: A, B, C, J, K, L, S, T, U
Length: 510 amino acids
Theoretical weight: 54.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07251 (Residues: 36-545; Coverage: 94%)
Gene names: ATP1, YBL0827, YBL099W
Sequence domains:
Structure domains:
ATP synthase subunit beta, mitochondrial Chains: D, E, F, M, N, O, V, W, X
Molecule details ›
Chains: D, E, F, M, N, O, V, W, X
Length: 484 amino acids
Theoretical weight: 52.01 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00830 (Residues: 36-511; Coverage: 93%)
Gene names: ATP2, J2041, YJR121W
Sequence domains:
Structure domains:
ATP synthase subunit gamma, mitochondrial Chains: G, P, Y
Molecule details ›
Chains: G, P, Y
Length: 278 amino acids
Theoretical weight: 30.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38077 (Residues: 34-311; Coverage: 89%)
Gene names: ATP3, ATP3a, ATP3b, YBR039W, YBR0408
Sequence domains: ATP synthase
Structure domains:
ATP synthase subunit delta, mitochondrial Chains: H, Q, Z
Molecule details ›
Chains: H, Q, Z
Length: 138 amino acids
Theoretical weight: 14.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12165 (Residues: 23-160; Coverage: 86%)
Gene names: ATP16, D2935, YD8119.03, YDL004W
Sequence domains: ATP synthase, Delta/Epsilon chain, beta-sandwich domain
ATP synthase subunit epsilon, mitochondrial Chains: 1, I, R
Molecule details ›
Chains: 1, I, R
Length: 61 amino acids
Theoretical weight: 6.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21306 (Residues: 2-62; Coverage: 98%)
Gene names: ATP15, P0345, YPL271W
Sequence domains: Mitochondrial ATP synthase epsilon chain
Structure domains: ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 110.43Å b: 288.58Å c: 187.17Å
α: 90° β: 101.37° γ: 90°
R-values:
R R work R free
0.213 0.212 0.259
Expression system: Saccharomyces cerevisiae