3ocf

X-ray diffraction
2.1Å resolution

Crystal structure of fumarate lyase:delta crystallin from Brucella melitensis in native form

Released:
Source organism: Brucella abortus 2308
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
L-aspartate = fumarate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate lyase:Delta crystallin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 478 amino acids
Theoretical weight: 51.46 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YLW1 (Residues: 11-466; Coverage: 94%)
Gene names: BAB1_1959, aspA
Sequence domains: Lyase
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 86.23Å b: 86.46Å c: 105.16Å
α: 90° β: 91.91° γ: 90°
R-values:
R R work R free
0.174 0.171 0.227
Expression system: Escherichia coli