› 3ob6

X-ray diffraction
3Å resolution

Structure of AdiC(N101A) in the open-to-out Arg+ bound conformation

Released:
DOI: 10.2210/pdb3ob6/pdb
PDB 3ob6 coloured by chain and viewed from the front.
PDB 3ob6 coloured by chain and viewed from the side.
PDB 3ob6 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Molecular basis of substrate-induced permeation by an amino acid antiporter.
Kowalczyk L, Ratera M, Paladino A, Bartoccioni P, Errasti-Murugarren E, Valencia E, Portella G, Bial S, Zorzano A, Fita I, Orozco M, Carpena X, Vázquez-Ibar JL, Palacín M
Proc Natl Acad Sci U S A 108 3935-40 (2011)
PMID: 21368142

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine/agmatine antiporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 46.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60061 (Residues: 1-445; Coverage: 100%)
Gene names: JW4076, adiC, aniC, b4115, yjdD, yjdE
Sequence domains: Amino acid permease

Ligands and Environments

1 bound ligand:
Ligand ARG 2 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 86.071Å b: 77.204Å c: 104.451Å
α: 90° β: 106.79° γ: 90°
R-values:
R R work R free
0.245 0.243 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Cationic amino acid transporters and their modulation by nitric oxide in cardiac muscle cells.
Peluffo RD. (2021)
13 more

14 mentions without citation

Insights into the Structure, Function, and Ligand Discovery of the Large Neutral Amino Acid Transporter 1, LAT1.
Singh et al. (2018)
13 more