3ob4

X-ray diffraction
2.71Å resolution

MBP-fusion protein of the major peanut allergen Ara h 2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAI domain-containing protein; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 55.66 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5Z1Q8 (Residues: 31-160; Coverage: 94%)
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
Gene names: Ara d 2.01, Ara h 2, JW3994, ara d 2, b4034, malE
Sequence domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 68.847Å b: 87.381Å c: 113.137Å
α: 90° β: 103.93° γ: 90°
R-values:
R R work R free
0.187 0.183 0.256
Expression system: Escherichia coli BL21(DE3)