› 3o9m

X-ray diffraction
2.98Å resolution

Co-crystallization studies of full length recombinant BChE with cocaine offers insights into cocaine detoxification

Released:
DOI: 10.2210/pdb3o9m/pdb
PDB 3o9m coloured by chain and viewed from the front.
PDB 3o9m coloured by chain and viewed from the side.
PDB 3o9m coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine.
Asojo OA, Asojo OA, Ngamelue MN, Homma K, Lockridge O
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 67 434-7 (2011)
PMID: 21505234

Function and Biology Details

Reaction catalysed: 
An acylcholine + H(2)O = choline + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholinesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 65.15 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06276 (Residues: 29-602; Coverage: 100%)
Gene names: BCHE, CHE1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P4212
Unit cell:
a: 150.392Å b: 150.392Å c: 142.448Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.262
Expression system: Cricetulus griseus

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Citations

3 mentions without citation

Plasma butyrylcholinesterase regulates ghrelin to control aggression.
Chen et al. (2015)
2 more