3o6v

X-ray diffraction
1.7Å resolution

Crystal structure of Uridine Phosphorylase from Vibrio cholerae O1 biovar El Tor

Released:
Entry authors: Maltseva N, Kim Y, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 27.96 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KT71 (Residues: 1-258; Coverage: 100%)
Gene name: VC_1034
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 93.074Å b: 93.074Å c: 155.569Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.148 0.178
Expression system: Escherichia coli