3o6c

X-ray diffraction
1.87Å resolution

Pyridoxal phosphate biosynthetic protein PdxJ from Campylobacter jejuni

Released:
Entry authors: Osipiuk J, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H(2)O
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxine 5'-phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.47 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PN59 (Residues: 1-257; Coverage: 100%)
Gene names: Cj1238, pdxJ
Sequence domains: Pyridoxal phosphate biosynthesis protein PdxJ
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I422
Unit cell:
a: 90.976Å b: 90.976Å c: 155.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.174
Expression system: Escherichia coli BL21(DE3)