› 3o2v

X-ray diffraction
2.3Å resolution

Crystal structure of 1E9 PheL89Ser/LeuH47Trp/MetH100bPhe, an engineered Diels-Alderase Fab with modified specificity and catalytic activity

Released:
DOI: 10.2210/pdb3o2v/pdb
PDB entry 3o2v coloured by chain, front view.
PDB entry 3o2v coloured by chain, side view.
PDB entry 3o2v coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Verdino P, Wilson IA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213341 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chimeric antibody Fab 1E9, light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Chimeric antibody Fab 1E9, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 227 amino acids
Theoretical weight: 24.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand TRS 10 x TRS
Ligand FLC 3 x FLC
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3121
Unit cell:
a: 128.73Å b: 128.73Å c: 92.342Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.219
Expression system: Escherichia coli

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