› 3o1q

X-ray diffraction
1.85Å resolution

Native Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF

Released:
DOI: 10.2210/pdb3o1q/pdb
PDB entry 3o1q coloured by chain, front view.
PDB entry 3o1q coloured by chain, side view.
PDB entry 3o1q coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Assembly of preactivation complex for urease maturation in Helicobacter pylori: crystal structure of UreF-UreH protein complex.
Fong YH, Wong HC, Chuck CP, Chen YW, Sun H, Wong KB
J Biol Chem 286 43241-9 (2011)
PMID: 22013070

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urease accessory protein UreF Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 254 amino acids
Theoretical weight: 28.65 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09065 (Residues: 1-254; Coverage: 100%)
Gene names: HP_0069, ureF
Sequence domains: UreF
Structure domains: Urease accessory protein UreF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 135.328Å b: 89.997Å c: 66.95Å
α: 90° β: 94.56° γ: 90°
R-values:
R R work R free
0.17 0.169 0.208
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Biosynthesis of the urease metallocenter.
Farrugia et al. (2013)
3 more

2 mentions without citation

Structural characterization of the urease accessory protein UreF from Klebsiella pneumoniae.
Liu et al. (2022)
1 more