3o03

X-ray diffraction
1.9Å resolution

Quaternary complex structure of gluconate 5-dehydrogenase from streptococcus suis type 2

Released:

Function and Biology Details

Reaction catalysed:
D-gluconate + NAD(P)(+) = 5-dehydro-D-gluconate + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase with different specificities (Related to short-chain alcohol dehydrogenase) Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.06 KDa
Source organism: Streptococcus suis 05ZYH33
Expression system: Escherichia coli
UniProt:
  • Canonical: A4VVQ2 (Residues: 1-271; Coverage: 100%)
Gene name: SSU05_1225
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I222
Unit cell:
a: 71.244Å b: 75.272Å c: 98.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.242
Expression system: Escherichia coli