› 3o01

X-ray diffraction
1.9Å resolution

The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Deoxycholate

Released:
DOI: 10.2210/pdb3o01/pdb
PDB 3o01 coloured by chain and viewed from the front.
PDB 3o01 coloured by chain and viewed from the side.
PDB 3o01 coloured by chain and viewed from the top.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.
Chatterjee S, Zhong D, Nordhues BA, Battaile KP, Lovell S, De Guzman RN
Protein Sci 20 75-86 (2011)
PMID: 21031487

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell invasion protein SipD Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 33.45 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56026 (Residues: 39-343; Coverage: 89%)
Gene names: STM2883, sipD, sspD
Structure domains: IpaD-like

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand DXC 1 x DXC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: C2
Unit cell:
a: 201.931Å b: 52.327Å c: 57.311Å
α: 90° β: 90.34° γ: 90°
R-values:
R R work R free
0.199 0.196 0.257
Expression system: Escherichia coli

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Citations

11 review citations

Type three secretion system in Salmonella Typhimurium: the key to infection.
Dos Santos et al. (2020)
10 more

2 mentions without citation

The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.
Chatterjee et al. (2011)
1 more