› 3nyu

X-ray diffraction
1.5Å resolution

X-ray crystal structure of the Wbpe (WlbE) aminotransferase from pseudomonas aeruginosa as the PLP internal aldimine adduct with lysine 185

Released:
DOI: 10.2210/pdb3nyu/pdb
PDB entry 3nyu coloured by chain, front view.
PDB entry 3nyu coloured by chain, side view.
PDB entry 3nyu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural investigation on WlaRG from Campylobacter jejuni: A sugar aminotransferase.
Dow GT, Gilbert M, Thoden JB, Holden HM
Protein Sci 26 586-599 (2017)
PMID: 28028852

Function and Biology Details

Reaction catalysed: 
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate = UDP-2-acetamido-2-deoxy-D-ribo-hex-3-uluronate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 367 amino acids
Theoretical weight: 40.26 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HZ76 (Residues: 1-359; Coverage: 100%)
Gene names: PA3155, wbpE
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand NA 2 x NA
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 47.628Å b: 54.068Å c: 150.903Å
α: 84.26° β: 82.43° γ: 64.93°
R-values:
R R work R free
0.183 0.181 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Investigation of a sugar N-formyltransferase from the plant pathogen Pantoea ananatis.
Hofmeister et al. (2019)
5 more

1 mention without citation

To Hit or Not to Hit, That Is the Question - Genome-wide Structure-Based Druggability Predictions for Pseudomonas aeruginosa Proteins.
Sarkar et al. (2015)