› 3ny5

X-ray diffraction
1.99Å resolution

Crystal structure of the RBD domain of serine/threonine-protein kinase B-raf from Homo sapiens. Northeast Structural Genomics Consortium Target HR4694F

Released:
DOI: 10.2210/pdb3ny5/pdb
PDB entry 3ny5 coloured by chain, front view.
PDB entry 3ny5 coloured by chain, side view.
PDB entry 3ny5 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Vorobiev S, Su M, Seetharaman J, Patel P, Xiao R, Ciccosanti C, Shastry R, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 96 amino acids
Theoretical weight: 11.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15056 (Residues: 153-237; Coverage: 11%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Raf-like Ras-binding domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 39.805Å b: 69.418Å c: 114.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.271
Expression system: Escherichia coli BL21(DE3)

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