3nwy

X-ray diffraction
2.54Å resolution

Structure and allosteric regulation of the uridine monophosphate kinase from Mycobacterium tuberculosis

Released:

Function and Biology Details

Reaction catalysed:
ATP + UMP = ADP + UDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 281 amino acids
Theoretical weight: 29.63 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHK5 (Residues: 1-261; Coverage: 100%)
Gene names: MTCY274.14c, Rv2883c, pyrH
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 136.71Å b: 175.48Å c: 65.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.269
Expression system: Escherichia coli BL21(DE3)