3nvi

X-ray diffraction
2.71Å resolution

Structure of N-terminal truncated Nop56/58 bound with L7Ae and box C/D RNA

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
NOP5/NOP56 related protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 379 amino acids
Theoretical weight: 43.94 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M1 (Residues: 1-369; Coverage: 92%)
Gene name: PF0060
Sequence domains: snoRNA binding domain, fibrillarin
Structure domains: Serum Albumin; Chain A, Domain 1
50S ribosomal protein L7Ae Chains: B, D
Molecule details ›
Chains: B, D
Length: 129 amino acids
Theoretical weight: 14.24 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U160 (Residues: 2-123; Coverage: 99%)
Gene names: PF1367, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
RNA (5'-R(*CP*UP*CP*UP*GP*AP*CP*CP*GP*AP*AP*AP*GP*GP*CP*GP*UP*GP*AP*UP*GP*AP*GP*C)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 24 nucleotides
Theoretical weight: 7.75 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 87.216Å b: 91.841Å c: 155.603Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.249
Expression system: Escherichia coli