3nqv

X-ray diffraction
1.7Å resolution

Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution R5 7/4A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
deoxyribose phosphate aldolase Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 28.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 90.524Å b: 64.725Å c: 49.032Å
α: 90° β: 104.69° γ: 90°
R-values:
R R work R free
0.206 0.205 0.234
Expression system: Escherichia coli