3npw

X-ray diffraction
2.14Å resolution

In silico designed of an improved Kemp eliminase KE70 mutant by computational design and directed evolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
deoxyribose phosphate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 28.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 52.386Å b: 82.872Å c: 127.964Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.259
Expression system: Escherichia coli