3npv

X-ray diffraction
1.48Å resolution

Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
deoxyribose phosphate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 27.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 62.595Å b: 53.518Å c: 81.702Å
α: 90° β: 110.34° γ: 90°
R-values:
R R work R free
0.145 0.143 0.17
Expression system: Escherichia coli