3nmv

X-ray diffraction
2.1Å resolution

Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2

Released:
Source organism: Arabidopsis thaliana

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Abscisic acid receptor PYL2 Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.1 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O80992 (Residues: 14-189; Coverage: 93%)
Gene names: At2g26040, PYL2, RCAR14, T19L18.15
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Protein phosphatase 2C 77 Chain: B
Molecule details ›
Chain: B
Length: 324 amino acids
Theoretical weight: 35.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O04719 (Residues: 101-423; Coverage: 76%)
Gene names: ABI2, At5g57050, MHM17.19, PP2C77
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 62.129Å b: 97.587Å c: 134.505Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.223
Expression system: Escherichia coli