3nmn

X-ray diffraction
2.15Å resolution

Crystal structure of pyrabactin-bound abscisic acid receptor PYL1 in complex with type 2C protein phosphatase ABI1

Released:
Source organism: Arabidopsis thaliana

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Abscisic acid receptor PYL1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 178 amino acids
Theoretical weight: 20.53 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VZS8 (Residues: 36-211; Coverage: 80%)
Gene names: At5g46790, MZA15.21, PYL1, RCAR12
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Protein phosphatase 2C 56 Chains: B, D
Molecule details ›
Chains: B, D
Length: 319 amino acids
Theoretical weight: 35.17 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P49597 (Residues: 117-434; Coverage: 73%)
Gene names: ABI1, At4g26080, F20B18.190
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 59.978Å b: 66.71Å c: 72.598Å
α: 115.78° β: 95.43° γ: 105.6°
R-values:
R R work R free
0.213 0.21 0.248
Expression system: Escherichia coli