3nk9

X-ray diffraction
1.65Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature

Released:
Source organism: Staphylococcus aureus
Primary publication:
Cavities determine the pressure unfolding of proteins.
Proc. Natl. Acad. Sci. U.S.A. 109 6945-50 (2012)
PMID: 22496593

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.12 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 31.075Å b: 60.435Å c: 38.129Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.186 0.181 0.224
Expression system: Escherichia coli BL21(DE3)