› 3nje

X-ray diffraction
1.85Å resolution

Structure of the Minor Pseudopilin XcpW from the Pseudomonas aeruginosa Type II Secretion System

Released:
DOI: 10.2210/pdb3nje/pdb
PDB entry 3nje coloured by chain, front view.
PDB entry 3nje coloured by chain, side view.
PDB entry 3nje coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system.
Franz LP, Douzi B, Durand E, Dyer DH, Voulhoux R, Forest KT
Acta Crystallogr D Biol Crystallogr 67 124-30 (2011)
PMID: 21245534

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein J Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 24.2 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00517 (Residues: 28-237; Coverage: 89%)
Gene names: PA3098, pddD, xcpW
Sequence domains: Type II secretion system (T2SS), protein J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 39.718Å b: 82.911Å c: 57.777Å
α: 90° β: 105.41° γ: 90°
R-values:
R R work R free
0.196 0.194 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
2 more

2 mentions without citation

Structural insights into the Type II secretion nanomachine.
McLaughlin et al. (2012)
1 more