PDBe 3ngo

X-ray diffraction
2.2Å resolution

Crystal structure of the human CNOT6L nuclease domain in complex with poly(A) DNA


Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
CCR4-NOT transcription complex subunit 6-like Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 45.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q96LI5 (Residues: 158-555; Coverage: 72%)
Gene names: CCR4B, CNOT6L
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
5'-D(*AP*AP*AP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 77.373Å b: 77.373Å c: 167.177Å
α: 90° β: 90° γ: 120°
R R work R free
0.217 0.215 0.263
Expression systems:
  • Escherichia coli
  • Not provided