3ngb

X-ray diffraction
2.68Å resolution

Crystal structure of broadly and potently neutralizing antibody VRC01 in complex with HIV-1 gp120

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Envelope glycoprotein gp160 Chains: A, D, G, I
Molecule details ›
Chains: A, D, G, I
Length: 353 amino acids
Theoretical weight: 39.21 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ED31 (Residues: 43-122, 201-303, 325-486; Coverage: 40%)
Gene name: env
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
Antigen binding fragment of heavy chain: Antibody VRC01 Chains: B, E, H, J
Molecule details ›
Chains: B, E, H, J
Length: 224 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antigen binding fragment of light chain: Antibody VRC01 Chains: C, F, K, L
Molecule details ›
Chains: C, F, K, L
Length: 210 amino acids
Theoretical weight: 23.17 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 108.63Å b: 98.275Å c: 205.256Å
α: 90° β: 99.68° γ: 90°
R-values:
R R work R free
0.2 0.197 0.256
Expression system: Homo sapiens