› 3ng1

X-ray diffraction
2.3Å resolution

N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS

Released:
DOI: 10.2210/pdb3ng1/pdb
PDB entry 3ng1 coloured by chain, front view.
PDB entry 3ng1 coloured by chain, side view.
PDB entry 3ng1 coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP.
Freymann DM, Keenan RJ, Stroud RM, Walter P
Nat Struct Biol 6 793-801 (1999)
PMID: 10426959

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 32.3 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: O07347 (Residues: 1-294; Coverage: 68%)
Gene name: ffh
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CD 6 x CD
Ligand SO4 2 x SO4
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 73.31Å b: 99.01Å c: 99.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.225
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The signal recognition particle.
Keenan et al. (2001)
14 more

1 mention without citation

Inhibition of SRP-dependent protein secretion by the bacterial alarmone (p)ppGpp.
Czech et al. (2022)