› 3nf4

X-ray diffraction
2.35Å resolution

Crystal structure of acyl-CoA dehydrogenase from Mycobacterium thermoresistibile bound to flavin adenine dinucleotide

Released:
DOI: 10.2210/pdb3nf4/pdb
PDB entry 3nf4 coloured by chain, front view.
PDB entry 3nf4 coloured by chain, side view.
PDB entry 3nf4 coloured by chain, top view.
Source organism: Mycolicibacterium thermoresistibile
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-CoA dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 41.62 KDa
Source organism: Mycolicibacterium thermoresistibile
Expression system: Escherichia coli
UniProt:
  • Canonical: G7CDN2 (Residues: 1-379; Coverage: 100%)
Gene name: KEK_05422
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I212121
Unit cell:
a: 95.65Å b: 99.23Å c: 171.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.24
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

3 mentions without citation

Mycobacterium tuberculosis utilizes a unique heterotetrameric structure for dehydrogenation of the cholesterol side chain.
Thomas et al. (2013)
2 more