› 3nek

X-ray diffraction
2.5Å resolution

Crystal structure of a nitrogen repressor-like protein MJ0159 from Methanococcus jannaschii

Released:
DOI: 10.2210/pdb3nek/pdb
PDB entry 3nek coloured by chain, front view.
PDB entry 3nek coloured by chain, side view.
PDB entry 3nek coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural underpinnings of nitrogen regulation by the prototypical nitrogen-responsive transcriptional factor NrpR.
Wisedchaisri G, Dranow DM, Lie TJ, Bonanno JB, Patskovsky Y, Ozyurt SA, Sauder JM, Almo SC, Wasserman SR, Burley SK, Leigh JA, Gonen T
Structure 18 1512-21 (2010)
PMID: 21070950

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Global nitrogen regulator NrpR Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.21 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57623 (Residues: 306-542; Coverage: 44%)
Gene names: MJ0159, nrpR
Sequence domains: NrpR regulatory domains NRD1 and 2
Structure domains: mj0159-like

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P3121
Unit cell:
a: 80.009Å b: 80.009Å c: 116.421Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.246 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.
Huergo et al. (2015)
1 more

1 mention without citation

Structural underpinnings of nitrogen regulation by the prototypical nitrogen-responsive transcriptional factor NrpR.
Wisedchaisri et al. (2010)