X-ray diffraction
3Å resolution

Crystal structure of a signal sequence bound to the signal recognition particle


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 19 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.38 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
  • Canonical: Q58440 (Residues: 1-87; Coverage: 100%)
Gene names: MJ1034, srp19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
Signal recognition particle 54 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 454 amino acids
Theoretical weight: 50.01 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Lactococcus lactis
  • Canonical: Q57565 (Residues: 3-431; Coverage: 95%)
Gene names: MJ0101, srp54
Sequence domains:
Structure domains:
SRP RNA Chain: M
Molecule details ›
Chain: M
Length: 136 nucleotides
Theoretical weight: 44.15 KDa
Sequence domains: Archaeal signal recognition particle RNA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 108.835Å b: 126.275Å c: 201.758Å
α: 90° β: 90° γ: 90°
R R work R free
0.23 0.228 0.267
Expression systems:
  • Escherichia coli
  • Lactococcus lactis