› 3nd2

X-ray diffraction
2.4Å resolution

Structure of Yeast Importin-beta (Kap95p)

Released:
DOI: 10.2210/pdb3nd2/pdb
PDB entry 3nd2 coloured by chain, front view.
PDB entry 3nd2 coloured by chain, side view.
PDB entry 3nd2 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Quantitative structural analysis of importin-β flexibility: paradigm for solenoid protein structures.
Forwood JK, Lange A, Zachariae U, Marfori M, Preast C, Grubmüller H, Stewart M, Corbett AH, Kobe B
Structure 18 1171-83 (2010)
PMID: 20826343

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Importin subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 861 amino acids
Theoretical weight: 94.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06142 (Residues: 1-861; Coverage: 100%)
Gene names: KAP95, L8300.15, YLR347C
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 58.835Å b: 128.272Å c: 69.05Å
α: 90° β: 102.68° γ: 90°
R-values:
R R work R free
0.215 0.213 0.267
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Nuclear import by karyopherin-βs: recognition and inhibition.
Chook et al. (2011)
9 more

8 mentions without citation

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
7 more