› 3nch

X-ray diffraction
2.88Å resolution

Yeast Glycogen Synthase (Gsy2p) Basal State Conformation

Released:
DOI: 10.2210/pdb3nch/pdb
PDB 3nch coloured by chain and viewed from the front.
PDB 3nch coloured by chain and viewed from the side.
PDB 3nch coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for glucose-6-phosphate activation of glycogen synthase.
Baskaran S, Roach PJ, DePaoli-Roach AA, Hurley TD
Proc. Natl. Acad. Sci. U.S.A. 107 17563-8 (2010)
PMID: 20876143

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen [starch] synthase isoform 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 725 amino acids
Theoretical weight: 82.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27472 (Residues: 1-705; Coverage: 100%)
Gene names: GSY2, L8479.8, YLR258W
Sequence domains: Glycogen synthase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand SO4 15 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 93.94Å b: 161.837Å c: 121.622Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.215 0.213 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Brain Glycogen Structure and Its Associated Proteins: Past, Present and Future.
Brewer et al. (2019)
9 more

2 mentions without citation

Structural basis for glucose-6-phosphate activation of glycogen synthase.
Baskaran et al. (2010)
1 more