3n9u

X-ray diffraction
1.92Å resolution

Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im

Released:
Source organism: Homo sapiens
Entry authors: Tresaugues L, Welin M, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Karlberg T, Kol S, Kotenyova T, Moche M, Nielsen TN, Nyman T, Persson C, Schuler H, Schutz P, Siponen MI, Thorsell AG, van der Berg S, Wahlberg E, Weigelt J, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cleavage and polyadenylation specificity factor subunit 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43809 (Residues: 21-227; Coverage: 91%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase
Cleavage and polyadenylation specificity factor subunit 7 Chains: C, I
Molecule details ›
Chains: C, I
Length: 156 amino acids
Theoretical weight: 17.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N684 (Residues: 50-182; Coverage: 28%)
Gene name: CPSF7
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 165.95Å b: 124.73Å c: 40.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.231
Expression system: Escherichia coli BL21(DE3)