3n8r

X-ray diffraction
1.41Å resolution

Crystal Structure of BlaC-E166A covalently bound with Carbenicillin

Released:
Source organism: Mycobacterium tuberculosis
Entry authors: Tremblay LW, Blanchard JS

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.21 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKD3 (Residues: 43-307; Coverage: 97%)
Gene names: MTCY49.07c, Rv2068c, blaA, blaC
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C, NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 49.631Å b: 67.856Å c: 75.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.182
Expression system: Escherichia coli