› 3n8h

X-ray diffraction
2Å resolution

Crystal Structure of Pantoate-beta-alanine Ligase from Francisella tularensis

Released:
DOI: 10.2210/pdb3n8h/pdb
PDB entry 3n8h coloured by chain, front view.
PDB entry 3n8h coloured by chain, side view.
PDB entry 3n8h coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Maltseva N, Kim Y, Papazisi L, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pantothenate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.21 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NF57 (Residues: 1-261; Coverage: 100%)
Gene names: FTT_1390, panC
Sequence domains: Pantoate-beta-alanine ligase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PRO 2 x PRO
Ligand AMP 2 x AMP
Ligand GOL 4 x GOL
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 47.2Å b: 47.2Å c: 259.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.223
Expression system: Escherichia coli

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