› 3n8b

X-ray diffraction
1.9Å resolution

Crystal Structure of Borrelia burgdorferi Pur-alpha

Released:
DOI: 10.2210/pdb3n8b/pdb
PDB entry 3n8b coloured by chain, front view.
PDB entry 3n8b coloured by chain, side view.
PDB entry 3n8b coloured by chain, top view.
Source organism: Borreliella burgdorferi
Primary publication:
Of bits and bugs--on the use of bioinformatics and a bacterial crystal structure to solve a eukaryotic repeat-protein structure.
Graebsch A, Roche S, Kostrewa D, Söding J, Niessing D
PLoS One 5 e13402 (2010)
PMID: 20976240

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF3276 family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.52 KDa
Source organism: Borreliella burgdorferi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O51076 (Residues: 3-100; Coverage: 80%)
Gene name: BB_0047
Sequence domains: Protein of unknown function (DUF3276)
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I212121
Unit cell:
a: 48.71Å b: 58.33Å c: 141.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The pur protein family: genetic and structural features in development and disease.
Johnson et al. (2013)
5 more

5 mentions without citation

Of bits and bugs--on the use of bioinformatics and a bacterial crystal structure to solve a eukaryotic repeat-protein structure.
Graebsch et al. (2010)
4 more