› 3n82

X-ray diffraction
2.25Å resolution

T244A mutant of Human mitochondrial aldehyde dehydrogenase, NADH complex

Released:
DOI: 10.2210/pdb3n82/pdb
PDB entry 3n82 coloured by chain, front view.
PDB entry 3n82 coloured by chain, side view.
PDB entry 3n82 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Gonzalez-Segura L, Hurley TD

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 500 amino acids
Theoretical weight: 54.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05091 (Residues: 18-517; Coverage: 97%)
Gene names: ALDH2, ALDM
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
4 bound ligands:
Ligand MG 8 x MG
Ligand NA 8 x NA
Ligand GAI 13 x GAI
Ligand EDO 24 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 142.216Å b: 150.724Å c: 177.299Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.219
Expression system: Escherichia coli BL21(DE3)

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