X-ray diffraction
2.21Å resolution

Structure of endothelial nitric oxide synthase N368D single mutant heme domain complexed with 4-(2-(5-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-3-yl)ethyl)-6-methylpyridin-2-amine

Source organism: Bos taurus

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nitric oxide synthase, brain Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 49.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
  • Canonical: P29476 (Residues: 280-293, 294-340, 341-711; Coverage: 30%)
Gene names: Bnos, Nos1
Structure domains:

Ligands and Environments

Cofactor: Ligand HEM 2 x HEM

Cofactor: Ligand H4B 2 x H4B

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 57.742Å b: 106.414Å c: 156.889Å
α: 90° β: 90° γ: 90°
R R work R free
0.189 0.187 0.238
Expression system: Escherichia coli