3n5f

X-ray diffraction
2.75Å resolution

Crystal Structure of L-N-carbamoylase from Geobacillus stearothermophilus CECT43

Released:

Function and Biology Details

Reaction catalysed:
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2)
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-carbamoyl-L-amino acid hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 44.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53389 (Residues: 1-408; Coverage: 100%)
Gene name: amaB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21212
Unit cell:
a: 103.208Å b: 211.683Å c: 43.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.276
Expression system: Escherichia coli BL21(DE3)