3n18

X-ray diffraction
1.6Å resolution

Crystal stricture of E145G/Y227F chitinase in complex with NAG from Bacillus cereus NCTU2

Released:

Function and Biology Details

Reaction catalysed:
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyco_18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 36.18 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VV09 (Residues: 28-360; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 48.683Å b: 75.073Å c: 79.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.227
Expression system: Escherichia coli