3mxw

X-ray diffraction
1.83Å resolution

Crystal structure Sonic hedgehog bound to the 5E1 fab fragment

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sonic hedgehog protein N-product Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15465 (Residues: 29-197; Coverage: 39%)
Gene name: SHH
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2
5E1 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
5E1 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 60.306Å b: 90.544Å c: 111.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.176 0.218
Expression system: Escherichia coli