X-ray diffraction
2.3Å resolution

The structure of GIY-YIG endonuclease R.Eco29kI


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Restriction endonuclease Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: Q46944 (Residues: 2-214; Coverage: 100%)
Gene name: eco29kIR
Sequence domains: Eco29kI restriction endonuclease

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1, ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 87.906Å b: 90.074Å c: 88.85Å
α: 90° β: 90° γ: 90°
R R work R free
0.185 0.183 0.228
Expression system: Escherichia coli