PDBe 3mqs

X-ray diffraction
2.4Å resolution

Crystal Structure of the USP7:Hdm2(PSTS) complex

Released:
Source organism: Homo sapiens
Entry author: Saridakis V

Function and Biology Details

Reactions catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 7 Chain: C
Molecule details ›
Chain: C
Length: 155 amino acids
Theoretical weight: 18.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93009 (Residues: 54-205; Coverage: 14%)
Gene names: HAUSP, USP7
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
E3 ubiquitin-protein ligase Mdm2 Chain: D
Molecule details ›
Chain: D
Length: 10 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q00987 (Residues: 394-403; Coverage: 2%)
Gene name: MDM2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P41
Unit cell:
a: 69.84Å b: 69.84Å c: 45.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided