› 3mpn

X-ray diffraction
2.25Å resolution

F177R1 mutant of LeuT

Released:
DOI: 10.2210/pdb3mpn/pdb
PDB entry 3mpn coloured by chain, front view.
PDB entry 3mpn coloured by chain, side view.
PDB entry 3mpn coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structural origins of nitroxide side chain dynamics on membrane protein α-helical sites.
Kroncke BM, Horanyi PS, Columbus L
Biochemistry 49 10045-60 (2010)
PMID: 20964375

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 56.76 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 5-511; Coverage: 99%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

5 bound ligands:
Ligand BOG 6 x BOG
Ligand LEU 1 x LEU
Ligand MTN 1 x MTN
Ligand CL 1 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 87.261Å b: 86.222Å c: 80.636Å
α: 90° β: 95.35° γ: 90°
R-values:
R R work R free
0.175 0.173 0.214
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Technological advances in site-directed spin labeling of proteins.
Hubbell et al. (2013)
4 more

6 mentions without citation

Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.
Muller et al. (2019)
5 more