3mnz

X-ray diffraction
1.8Å resolution

Crystal structure of the non-neutralizing HIV antibody 13H11 Fab fragment with a gp41 MPER-derived peptide bearing Ala substitutions in a helical conformation

Released:
Entry authors: Nicely NI, Dennison SM, Kelsoe G, Liao H-X, Alam SM, Haynes BF

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 21 amino acids
Theoretical weight: 2.33 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 653-671; Coverage: 2%)
Gene name: env
ANTI-HIV-1 ANTIBODY 13H11 LIGHT CHAIN Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.72 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
ANTI-HIV-1 ANTIBODY 13H11 HEAVY CHAIN Chain: B
Molecule details ›
Chain: B
Length: 219 amino acids
Theoretical weight: 23.52 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 66.89Å b: 121.692Å c: 58.097Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.215
Expression systems:
  • Not provided
  • Homo sapiens