› 3mnp

X-ray diffraction
1.5Å resolution

Crystal structure of the agonist form of mouse glucocorticoid receptor stabilized by (A611V, V708A, E711G) mutations at 1.50A

Released:
DOI: 10.2210/pdb3mnp/pdb
PDB entry 3mnp coloured by chain, front view.
PDB entry 3mnp coloured by chain, side view.
PDB entry 3mnp coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening.
Seitz T, Thoma R, Schoch GA, Stihle M, Benz J, D'Arcy B, Wiget A, Ruf A, Hennig M, Sterner R
J Mol Biol 403 562-77 (2010)
PMID: 20850457

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139079 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucocorticoid receptor Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 30.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06537 (Residues: 536-792; Coverage: 32%)
Gene names: Grl, Grl1, Nr3c1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.59 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q61026 (Residues: 740-752; Coverage: 1%)
Gene names: Grip1, Ncoa2, Src2, Tif2

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand DEX 1 x DEX
Ligand SCN 2 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P65
Unit cell:
a: 72.089Å b: 72.089Å c: 128.827Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 0.201
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Modulating protein stability - directed evolution strategies for improved protein function.
Socha et al. (2013)
3 more

5 mentions without citation

The Biologist's Guide to the Glucocorticoid Receptor's Structure.
Deploey et al. (2023)
4 more