› 3mnf

X-ray diffraction
2.97Å resolution

Crystal structure of PAC2 family protein from Streptomyces avermitilis MA

Released:
DOI: 10.2210/pdb3mnf/pdb
PDB entry 3mnf coloured by chain, front view.
PDB entry 3mnf coloured by chain, side view.
PDB entry 3mnf coloured by chain, top view.
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Entry authors: Chang C, Hatzos C, Morgan T, Bearden J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylate--amine ligase Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.38 KDa
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q828L9 (Residues: 11-257; Coverage: 74%)
Gene names: SAVERM_6650, parJ
Sequence domains: PAC2 family
Structure domains: PAC-like subunit

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 143.943Å b: 143.943Å c: 81.121Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.216 0.241
Expression system: Escherichia coli BL21(DE3)

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