X-ray diffraction
2Å resolution

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation


Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-5C Chains: A, F, K
Molecule details ›
Chains: A, F, K
Length: 374 amino acids
Theoretical weight: 41.72 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
  • Canonical: P10987 (Residues: 3-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:
Protein spire Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 19 amino acids
Theoretical weight: 1.64 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 125.3Å b: 55.95Å c: 125.37Å
α: 90° β: 120.04° γ: 90°
R R work R free
0.193 0.191 0.239
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli