3ml1

X-ray diffraction
1.6Å resolution

Crystal Structure of the Periplasmic Nitrate Reductase from Cupriavidus necator

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic nitrate reductase Chain: A
Molecule details ›
Chain: A
Length: 802 amino acids
Theoretical weight: 90.51 KDa
Source organism: Cupriavidus necator H16
Expression system: Cupriavidus necator
UniProt:
  • Canonical: P39185 (Residues: 30-831; Coverage: 100%)
Gene names: PHG211, napA
Sequence domains:
Structure domains:
Periplasmic nitrate reductase, electron transfer subunit Chain: B
Molecule details ›
Chain: B
Length: 135 amino acids
Theoretical weight: 15.39 KDa
Source organism: Cupriavidus necator H16
Expression system: Cupriavidus necator
UniProt:
  • Canonical: P39186 (Residues: 35-169; Coverage: 100%)
Gene names: PHG212, napB

Ligands and Environments


Cofactor: Ligand MGD 2 x MGD

Cofactor: Ligand HEC 2 x HEC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 142.202Å b: 82.384Å c: 96.837Å
α: 90° β: 100.72° γ: 90°
R-values:
R R work R free
0.159 0.157 0.19
Expression system: Cupriavidus necator