3mki

X-ray diffraction
2Å resolution

Crystal Structure of Ketosteroid Isomerase D38ED99N from Pseudomonas Testosteroni (tKSI)

Released:
Source organism: Comamonas testosteroni
Entry authors: Gonzalez A, Tsai Y, Schwans J, Sunden F, Herschlag D

Function and Biology Details

Reaction catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 125 amino acids
Theoretical weight: 13.43 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00947 (Residues: 1-125; Coverage: 100%)
Gene name: ksi
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1, SSRL BEAMLINE BL9-2
Spacegroup: P6122
Unit cell:
a: 64.041Å b: 64.041Å c: 502.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.213
Expression system: Escherichia coli BL21